#SpectraMax L Config) Methods. Unparalleled performance on a personalized platform. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. CO 2 & O 2 control. Create custom fluorescence assays without having to purchase expensive filter sets. 012 fmol 0. Plus 384. Selects either 96-well or 384-well plate for a stand-alone read. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. Reads various kinds of 96-wells plates, and is equipped with shaking function. Three low volume 24-well bottom slides. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. Gemini XPS Reader scans. May require LS-50B…. Compare this item. Corning. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. x. Figure 2-23: Bottle holder To install the bottle holder: 1. x. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. a white 96-well half-area microplate (Corning cat. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. An example of dual-luciferase assay results. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 300 µL of Detergent Compatible Bradford reagent was added to each well. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. Figure 1. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. Shaking: Linear, orbital, and double orbital. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 00 USD. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. * Note: Programming the washer with a final aspirate height of 4. 000 OD ± 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. 0025 OD from 0. 340 PC384. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. A control well was set up to verify. Make sure toPlate reader instructions Computer. These robust readers have been placed in labs from Antarctica to. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. x. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. #3693; n = 4 for each standard). Related Products: 96 Well Plate Reader. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 96-well-plate-scan-time-2-colors-1-site-well. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. Packed 25 plates per sleeve, 2 sleeves per case. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Growth medium was then replaced with fatty acid uptake solution. • Easy and gentle immobilization of biotin. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Our. VMax . Clear plates will give more cross talk between wells. variety of. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Contact our support team with your questions regarding Molecular Devices products and applications. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Specifications subject to change without notice. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. x. (Molecular Devices cat. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #CA961) Methods. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. #ABS Plus) Cell plating and treatment prior to assay. Instrument settings can. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Linear, Orbital Slow, and Orbital Fast. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. White 96-well platePlate formats. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. SpectraDrop High-Throughput Screening (HTS) Kit. 1 fmol luciferase per well. Part Number. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. Absorbance equation. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. 96- and 384-well microplates. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Plate chamber design of the SpectraMax category 1 microplate readers. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 2. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. 306 plate air cal. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. The picked. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 1 OD at 230 nm. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Dynamic range > 6 decades . Now select the area of the plate to read. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. % transmittance of plates used in a plate reader. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. . Cross-talk <0. 303 unable to cal dark current. Service parts for these models are not being produced and are no longer in stock. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Dimensions: 18. UV absorbance detection. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. 5% at 1. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 2b,c. 1. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. Fluorescence Microplate Reader Comparison Chart. Intuitive graphical user interface makes each assay extremely easy to set. Published Dec 16, 2020 | Updated Dec 16, 2020. flat-bottom: 50-60 µl. x. x. 384-WELL SBS STERILE MICROPLATE. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Four wash/rinse bottles are included and configurable for both 96- and 384. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. Washers configured for both 96- and 384-well plates. VMax . What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Published Dec 16, 2020 | Updated Dec 16, 2020. 45 µm PVDF. explore more. Supersedes 1. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Ensure that cells reach around 80–90% of confluence on the day of the. White 96-well plate Plate formats. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Polypropylene deep well plates, round bottom. 314 low lamp level warning. Range of 96, 384-well SBS microplates . 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. No accessories are needed and SoftMax® Pro Software. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 00 USD. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. x. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. TNF standard curve. 0 . The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. QuickSync. 1. Bay Gray powder coated metal back plate, column, and base. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 0 . d. 96-, 384-, and 1536-well microplates. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. #C1500) Control standard endotoxin (ACC cat. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 01. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. minutes and begin reading microplates with seven mouse clicks. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Published Dec 16,. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Used. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Therefore you need to ask a physicist. Products : Ionworks HT, Ionworks Quattro. Click on the crosshair and move it so that it is centered over the well image. Running both standard spectrophotometer and microplate. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SpectraMax 250 User. *For Corning UV plates, mean values at 260 nm ranged from 0. Service Contracts, Preventative Maintenance and. All trademarks used herein are the property of. External Dimensions without lid (mm). Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Reader Documentation Version No. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 1. (96- or 384-well) simultaneously and with high temporal resolution. maximum-illumination-channels. UV absorbance detection. 5 ng per well. Two different agonists, acetylcholine and carbachol, were tested. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. $488. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Top or bottom detection is available with a simple click of a button. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. 96-well plates - Filter plates with 0. 1. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. 00. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Select up to 3 products. 5 fg per well to 0. 300 µL of Detergent Compatible Bradford reagent was added to each well. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 100 fmol 384-well (75 µL) 0. 3 OD at 200 nm. 046 to 0. 96-well) while others have flexibility to. For biochemical assays, the washer removes unbound material and unreacted reagents. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Published Dec 16, 2020 | Updated Dec 16,. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 1 mL/well of media. x. 96-, 384-, and 1536-well microplates. Or scan up to 384 wells at any time to determine the best assay conditions. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. 7°C and the bottom to 36. 96- and 384-well. White plates reflect the light, enabling even low levels of Luminescence to be detected. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Figure 3. These robust readers have been placed in labs from Antarctica to the. 304 signal level saturation. Part of a plate can be selected so that only a selected area of the plate. When a. 96/384-well plate formats. The system can perform readings. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Molecular Devices L-Max II Luminescence Microplate Reader. 00 . Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Compare Tool. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. The soil was amended with either 1. Access this by going to. Integrated stacker option. 340 PC384. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. The equation that allows one to calculate absorbance from % transmittance is. Microplate Readers include the M3, M4, M5, and M5e models. Gemini EM Reader scans. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. g. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 0 nm increments Dynamic range > 7 decades Cross-talk < 0. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. FlexIII. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Absorbance Linearity: 2. BMG Labtech - THERMOstar. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. #N207) LAL Reagent Water (Lonza cat. The system features 96, 384,. They can be read by most microplate readers. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The Versamax Microplate Reader is built around a grating monochromator. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. 00. Making it possible to perform kinetic analysis of solid. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. SoftMax Pro GxP Software version 5. QC tools and IQOQ services. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. Microplate Reader. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Contents of wells were pipetted up and down several times to mix. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The VICTOR Nivo. 305 reference level saturation. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Detection of KASP genotyping reactions. Compare this item. Highly selective for dsDNA over ssDNA or RNA. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. x. x. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). This can increase throughput and efficiency, thus saving time and space in the lab. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. 17,000 cells. Published Dec 16, 2020 | Updated Dec 16, 2020. NanoQuant plate. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. . 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research.